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KMID : 0375320000220020235
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Journal of Clinical Pathology and Quality Control
2000 Volume.22 No. 2 p.235 ~ p.241
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Influence of Iron Depletion on Immature Reticulocyte Fractions and Reticulocyte Maturity Index
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Choi Jong-Won
Pai Soo-Hwan
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Abstract
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Background : To investigate the influence of body iron status on immature reticulocyte fractions and reticulocyte maturity index (RMI), we measured serum iron markers, fluorescent intensity of reticulocytes, and serum transferrin receptor (sTfR) concentrations in 374 female adolescents.
Method : Reticulocytes and their fractions were automatically analyzed by flow cytometry (R3000; Sysmex, Kobe, Japan). Serum iron and total iron-binding capacity (TIBC) were assayed with the automatic chemical analyzer (Hitachi 747; Hitachi, Tokyo, Japan) and ferritin was measured by the chemiluminescence method (ACS 180; Chiron, USA). Soluble transferrin receptor was measured by immunoenzymometric method (Orion Diagnostica, Finland).
Results: There were no significant differences in the values of reticulocyte fractions and RMI between ferritin alone-depleted group and healthy controls. However, middle- and highfluorescence reticulocytes´ (MFR and HFR), and RMI were significantly higher in both serum iron and serum ferritin depleted group than in ferritin alone-depleted group. MFR and RMI increased gradually as body iron store was depleted and were 3.4- and 3.6-fold higher, respectively than normal controls, when the subjects attained a frank iron deficiency anemia. There were no significant changes in the values of red blood cells and total reticulocyte counts during irondepleted states. Mean value of sTfR (3.98 mg/L) in the subjects with RMI > 1.5% was significantly higher than that (2.26 mg/L) in the subjects with RMI < 1.5% (p < 0.01). The sTfR concentration correlated significantly with RMI (r = 0.61, p < 0.01) and MFR (r = 0.59, p < 0.01).
Conclusions : Body iron depletion induces to elevate immature reticulocyte fractions and RMI. Increase of reticulocyte fluorescent intensity during iron-depleted states seems to be influenced by an increased amount of intracellular TfR RNA.
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KEYWORD
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Iron status, Reticulocyte fractions, Reticulocyte maturity index, Transferrin receptor
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